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General Forums - Note: You must Be Logged In to post. Anyone can browse. => Mitochondrial DNA => Topic started by: Trish13 on July 10, 2013, 09:22:42 AM



Title: Please could I have help with Mum's result? Thanks!
Post by: Trish13 on July 10, 2013, 09:22:42 AM
Hi :)

I have had a lot of very useful help from the knowledgeable members of this forum and now I need help, please, with my mother's mt DNA result.

My Mum has has had her mitochondrial DNA tested by FT DNA. She has been given the Haplogroup - H

Here is more of the info they gave:


RSRS Values

HVR1 Differences from RSRS:

T16187C
C16189T
T16223C
G16230A
T16278C
C16311T
A16316G


HVR2 Differences from RSRS:

G73A
C146T
C152T
C195T
A247G
309.1C
315.1C
522.1A
522.2C   

rCRS Values:

HVR1 DIFFERENCES FROM rCRS:

16129A
16316G
16519C

HVR2 DIFFERENCES FROM rCRS:
263G
309.1C
315.1C


HVR1 REFERENCE SEQUENCE

Position   CRS   Your Result
16129      G   A
16316      A   G
16519      T   C

HVR2 REFERENCE SEQUENCE

Position   CRS   Your Result
263      A           G
309.1          C
315.1          C


I don't know quite what I can learn from this.

I have now received a message from FT DNA:

'A mitochondrial DNA hypervariable region 1 and 2 (mtDNA HVR1 & HVR2) match has been found ... in the Family Tree DNA database' but i have no idea whether this is an important match or not.

Could such a match be important, or do I need more mtDNA testing in order to get useful results?

Thank you! :)


Title: Re: Please could I have help with Mum's result? Thanks!
Post by: Maliclavelli on July 10, 2013, 09:48:09 AM
Your match is this:
First Name: Julia
 Last Name: Stout
Year Born: About 1800
Year Died: About 1846
 Country of Origin: North Carolina, USA

 
Contact person:   William L. Shook Contact this user            

But also these:
 
Haplogroup:   H               
Tested with:   Family Tree DNA               
Contact person:   Dianne Plunkett Latham Contact this user            

Most distant known maternal ancestor on the direct female line
 
First Name:   Patience      
Last Name:   Mrs. James Campbell      
Year Born:         
Year Died:   1804      
Country of Origin:   Northern Ireland    

Most distant known maternal ancestor on the direct female line
 
First Name:   Nancy      
Last Name:   Harvill      
Year Born:   About 1820      
Year Died:   About 1880      
Country of Origin:   Unknown, United Kingdom   

Search for Genetic Matches > Enter Search Parameters > Search Results > Displaying User

 
   Mutations            
   HVR1      HVR2      
1.   16129A      00263G      
2.   16316G      00309.1C      
3.   16519C      00315.1C   
 
Haplogroup:   H*               
Tested with:   Family Tree DNA               
Contact person:   Gray Carpenter Church Contact this user            

Most distant known maternal ancestor on the direct female line
 
First Name:   Nancy Elizabeth      
Last Name:   Overstreet      
Year Born:   1867      
Year Died:   1925      
Country of Origin:   Unknown    
 
ogroup:   H               
Tested with:   Family Tree DNA               
Contact person:   Jack Turner Contact this user            

Most distant known maternal ancestor on the direct female line
 
First Name:   Stina Sophia      
Last Name:   Kaarlsdotter      
Year Born:   1833      
Year Died:   1901      
Country of Origin:   Tjarstad, Ostergotland, Sweden   

and many many others.



Title: Re: Please could I have help with Mum's result? Thanks!
Post by: Maliclavelli on July 10, 2013, 09:56:18 AM
It seems it is H27. Anyway for being sure and finding the closest matches you should test the coding region, i.e. do an FGS.


Title: Re: Please could I have help with Mum's result? Thanks!
Post by: Trish13 on July 10, 2013, 10:24:24 AM
Thank you for your help, Maliclavelli. You're very kind!

I'm even less knowledgeable on mtDNA than I am on Y-DNA, but I am keen to understand more.

As you say, there are a number of matches, but how can I tell whether or not they are genuinely close and useful ones?

I don't know what 'FGS' is. I'll have a look on the Internet for more information.

Thanks again :)


Title: Re: Please could I have help with Mum's result? Thanks!
Post by: Maliclavelli on July 10, 2013, 10:38:56 AM
Thank you for your help, Maliclavelli. You're very kind!

I'm even less knowledgeable on mtDNA than I am on Y-DNA, but I am keen to understand more.

As you say, there are a number of matches, but how can I tell whether or not they are genuinely close and useful ones?

I don't know what 'FGS' is. I'll have a look on the Internet for more information.

Thanks again :)
This will be my 2000 post, and is for you, Trish 13.
FGS was the previous name (now they use others) and does mean Full Genome Sequence, i.e. the reading of all the mitochondrion: 16569 bases. Having you had the sequence of HVRI and II, this is the only upgrade you may do. Perhaps they call it  HVRI and II mega or something similar.


Title: Re: Please could I have help with Mum's result? Thanks!
Post by: Trish13 on July 11, 2013, 01:25:59 PM
Hi Maliclavelli :)

I'm honoured :)

And I understand, now, about FGS, but the next step in testing is quite a bit more expensive, so I'll have to wait awhile :)

Thanks again!


Title: Re: Please could I have help with Mum's result? Thanks!
Post by: Maliclavelli on July 11, 2013, 02:55:01 PM
Hi Maliclavelli :)

I'm honoured :)

And I understand, now, about FGS, but the next step in testing is quite a bit more expensive, so I'll have to wait awhile :)

Thanks again!

We paid for this test 289$. Now you may spend, with this offering, 100$ less. It is a good price, because the test is complex: 16569 nucleotides and it is the definite one. Anyway I understand it could be expensive, but, if one wants to know, this is the way. You know this also for the future. Hope that price may diminish yet.


Title: Re: Please could I have help with Mum's result? Thanks!
Post by: Trish13 on July 31, 2013, 03:20:44 PM
Hi again :)

Sorry, I missed this until now. :)

Prices are certainly coming down, which is great. It's just that I am trying to get at least basic results on all of the possible lines of my great grandparents ~ and costs mount up.  

I'm following as many Mitochondrial and Y lines as I can ~ plus an autosomal test on my brother's DNA.

So far, re mtDNA, as well as this 'H' result (my mother), I have a 'V' (paternal great grandmother's line) and a 'K' (maternal great grandmother's line).

But you are right, I feel that, without the full test, I am unlikely to be able to go any further with this.


Title: Re: Please could I have help with Mum's result? Thanks!
Post by: Trish13 on November 23, 2014, 10:31:33 PM
After my brother's Geno 2 test and autosomal testing we have now refined the mitochondrial result for my mother's line: H27c (as Maliclavelli predicted) :)