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Please Note that these markers are displayed in strict numerical order.

These results do NOT include the Kittler test results. These are shown at Bobs DNA I2* site

Oldest Known Relation DYS DYF  DYF  DYF  DYF  DYF  DYF  DYF  DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS DYS
  I2* S31 - ADR 156-Y 371X 385 397 399X 401 408 411 434 435 464x 485 494 495 505 522 533 549 556 575 589 636 638 643 710 714 716 717 725 726
                                                                                                       
                                                                                                       
  I2* S31 - A  
11714 William Livingston, b: 1793 Burke/Jefferson Co GA  9 10c 10c 12t 13c 10 10 14 14 14 14 21t 21r 17c 17 17 188 188 10 12 12 13 9 11 11g 15g 16g 17g 14 9 14 11 11 12 11 11 8 12 12 10 12 29 24 27 19 31 31 32 32 12
103209 Lockhart                        20t 21t 25c                                                                        
                                                                                                       
  I2* S31 - B  
163679 Arthur Spriggs 1876 - 1960                                                                                  12                  
155679 Pr. Hasan Jalal Dawla, 13th C., Karabagh                        21t 25c 25c                                                                        
182630 Safarians, Karadagh, Iran                        17c 21t 25c                                                                        
N61682 Nishan Toumajan, 1893-1989, Gurun, Turkey                        24c 24c 24t                                                                        
                                                                                                       
  I2* S31 - B (J)  
                                                                                                       
                                                                                                       
  I2* S31 - C  
                                                                                                       
                                                                                                       

 

Useful Links:

This article gives a detailed description of  marker DYS 464

Recombinational Loss of Heterozygosity (recLOH)

 

The DYS464X (Extended) Test

The original report can be found at the following link. I is copied here for your convenience.

http://www.dna-fingerprint.de/modules.php?op=modload&name=Sections&file=index&req=viewarticle&artid=11

Definition of DYS464X
DYS464X is a special method of testing the DYS464 STR alleles. R1b males have a G/C SNP in some of their DYS464 flanking regions very close to the reverse primer of the DYS464 locus. The DYS464X test uses altered primer sequences that end with their last base exactly at the SNP. One primer ends with a "G", while the other primer ends with a "C". These primers allow us to test the different SNP variants and their corresponding DYS464 STR alleles separately.
If the primer ending with G yields a peak, the allele is classed as a G-type.
If the primer ending with C yields a peak, the allele is classed as a C-type.

DYS464-forward

FAM-ttacgagctttgggctatg (exactly from the Redd paper)

DYS464-backward-G-Type

JOE-cctgggtaacagagagactctttcag (ending with G)

DYS464-backward-C-Type

TMR-cctgggtaacagagagactctttcac (ending with C)

PCR parameters: 30 x (90°, 65°, 70°) and 60° end-elongation 1/2 h
Peaks with a green fluorescence (JOE) are called G type.
Peaks with a yellow fluorescence (TMR) are called C type.
There are some sample electropherograms in my library:

http://www.dna-fingerprint.com/index.php?module=photoshare&func=showimages&fid=3
If you want to see a sequence alignment of the DYS464 DNA sequences and the primers you can download a BioEdit file from my alignment library:
[DYS464 Sequence Alignment]
The BioEdit alignment editor for Windows software can be downloaded from here:
http://www.mbio.ncsu.edu/BioEdit/
What is the use of the DYS464X test?
Most R1b males that we have tested show 3 C-types and 1 G-type.
All other haplogroups (including R1a) show 4 G-types.

1.)

The DYS464X method can verify, if a person is really R1b

2.)

In many cases we can determine, if a single large peak really represents two different alleles

3.)

Some DYS464 patterns look similar when the conventional test is used, but really consist of completely different alleles. For example:
14c-15c-17c-17g is completely different from
14c-15g-17c-17c,
but this wouldn't be seen with the conventional typing method.

4.)

DYS464X is a method that helps us observe recombinational loss of heterozygosity (recLOH)

Some DYS464 examples from an arbitary surname project:
(conventional results)
surname A, 1: 15-15-16-17
surname A, 2: 15-15-16-17
surname A, 3: 15-15-16-17
surname B, 1: 15-15-16-18
surname C, 1: 15-15-15-17
If all the 5 persons are R1b then they can be typed with an extended DYS464 test.
We might get the following results:
surname A, 1: 15c-15c-16g-17c
surname A, 2: 15c-15c-16g-17c
surname A, 3: 15c-15c-16c-17g
surname B, 1: 15c-15c-16g-18c
surname C, 1: 15c-15c-15g-17c
From that result we could say:
- A1 and A2 match completely
- A3 has at least a genetic distance of 2 from (A1 & A2)
- B1 has a genetic distance of 1 from (A1 & A2)
- C1 has a genetic distance of 1 from (A1 & A2)
I hope this is not too technical! Feel free to ask questions.

Thomas Krahn

 

 


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