Please Note that these markers are displayed in strict numerical order.

These results do NOT include the Kittler test results. These are shown at Bobs DNA I2* site

Useful Links:

This article gives a detailed description of  marker DYS 464

Recombinational Loss of Heterozygosity (recLOH)

The DYS464X (Extended) Test

The original report can be found at the following link. I is copied here for your convenience.

http://www.dna-fingerprint.de/modules.php?op=modload&name=Sections&file=index&req=viewarticle&artid=11

Definition of DYS464X
DYS464X is a special method of testing the DYS464 STR alleles. R1b males have a G/C SNP in some of their DYS464 flanking regions very close to the reverse primer of the DYS464 locus. The DYS464X test uses altered primer sequences that end with their last base exactly at the SNP. One primer ends with a "G", while the other primer ends with a "C". These primers allow us to test the different SNP variants and their corresponding DYS464 STR alleles separately.
If the primer ending with G yields a peak, the allele is classed as a G-type.
If the primer ending with C yields a peak, the allele is classed as a C-type.

PCR parameters: 30 x (90°, 65°, 70°) and 60° end-elongation 1/2 h
Peaks with a green fluorescence (JOE) are called G type.
Peaks with a yellow fluorescence (TMR) are called C type.
There are some sample electropherograms in my library:
http://www.dna-fingerprint.com/index.php?module=photoshare&func=showimages&fid=3
If you want to see a sequence alignment of the DYS464 DNA sequences and the primers you can download a BioEdit file from my alignment library:
[DYS464 Sequence Alignment]
The BioEdit alignment editor for Windows software can be downloaded from here:
http://www.mbio.ncsu.edu/BioEdit/
What is the use of the DYS464X test?
Most R1b males that we have tested show 3 C-types and 1 G-type.
All other haplogroups (including R1a) show 4 G-types.

Some DYS464 examples from an arbitary surname project:
(conventional results)
surname A, 1: 15-15-16-17
surname A, 2: 15-15-16-17
surname A, 3: 15-15-16-17
surname B, 1: 15-15-16-18
surname C, 1: 15-15-15-17
If all the 5 persons are R1b then they can be typed with an extended DYS464 test.
We might get the following results:
surname A, 1: 15c-15c-16g-17c
surname A, 2: 15c-15c-16g-17c
surname A, 3: 15c-15c-16c-17g
surname B, 1: 15c-15c-16g-18c
surname C, 1: 15c-15c-15g-17c
From that result we could say:
- A1 and A2 match completely
- A3 has at least a genetic distance of 2 from (A1 & A2)
- B1 has a genetic distance of 1 from (A1 & A2)
- C1 has a genetic distance of 1 from (A1 & A2)
I hope this is not too technical! Feel free to ask questions.
Thomas Krahn